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当前位置: 首页 > 产品中心 > Sandwich_method_ELISA > Biosensis/NGFR/p75ECD Rapid ELISA Kit: Human (2 Plates)/BEK-2219-2P/1 Ea
商品详细Biosensis/NGFR/p75ECD Rapid ELISA Kit: Human (2 Plates)/BEK-2219-2P/1 Ea
Biosensis/NGFR/p75ECD Rapid ELISA Kit: Human (2 Plates)/BEK-2219-2P/1 Ea
Biosensis/NGFR/p75ECD Rapid ELISA Kit: Human (2 Plates)/BEK-2219-2P/1 Ea
商品编号: BEK-2219-2P
品牌: Biosensis inc
市场价: ¥15340.00
美元价: 9204.00
产地: 美国(厂家直采)
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产品分类: 夹心法ELISA
公司分类: Sandwich_method_ELISA
联系Q Q: 3392242852
电话号码: 4000-520-616
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品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
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DescriptionTheBiosensisNGFR/p75ECDRapidTMenzyme-linkedimmunosorbentassay(ELISA)KitisasandwichELISAthatallowsthequantificationoftheextracellulardomain(ECD)ofhumanp75neurotrophinreceptorinlessthan4hoursinurine,serum,citrate-plasma,CSFandcellculturesupernatantsonlyifusedasdirected.Pleaserefertothekitprotocolforspecificuseinstructionsforeachsubstrateapplication.

Notethatcaptureanddetectionantibodieshavebeenoptimizedforincreasedassaysensitivity,andreagentsfrompreviouslotsshouldnotbeused.Thisoptimizedhumanp75ECDELISAassaydemonstratesanincreasedsensitivity,loweringtheLODabout8-foldtothepreviousassayversion.ItrepresentsthemostsensitiveNGFR/p75ECDELISAassayavailabletoday,withadetectionlimitofatleast6pg/mL,andaquantificationrangeof7.8-500pg/mL.

ThisELISAkitconsistsofapre-coatedmousemonoclonalanti-p75ECDcaptureantibody,abiotinylatedmousemonoclonalanti-p75ECDdetectionantibodyandhorserADIshperoxidase(HRP)-conjugatedstreptavidin.Theadditionofasubstrate(3,3’,5,5’-tetramethylbenzidine,TMB)yieldsacoloredreactionproductwhichisdirectlyproportionaltotheconcentrationofp75ECDpresentinsamplesandproteinstandards.Ahumanp75ECDpositivecontrol(QCsample)isprovidedtoassureconsistentassayperformance.

ThisNGFR/p75ECDELISAkitemploysanin-houserecombinanthumanp75ECD-Fcchimeraasstandardthathasbeenvalidatedagainstanexternallyavailablep75ECD-Fcprotein.Whilethereisacurrentlackofacommerciallyavailable,truehumanp75ECDstandard,thisELISAkitallowsquantificationofhumanp75ECDasp75ECD-Fchumanequivalents.PleasenotethattheantibodiesusedinthisELISAdonotcross-reactwithmousep75ECD.

Note1:Forresearchuseonly.Notfordiagnosticandclinicalpurposes.
Note2:ThisELISAshouldnotbeconfusedwiththatreportedinthePLoSONEpublicationbyShepheardetal(2014,2017)oranyotherassay.Thiskitisuniqueandhasbeendeveloped,manufacturedandextensivelyvalidatedin-houseandisthemostsensitiveELISAavailableforthedetectionofp75NTR-ECDinhumansamples.
RelatedproductsBEK-2220-2P,MouseNGFR/p75ECDRapidTMELISA

BL-002-1250,HeterophilicantibodyblockerforELISAassaysutilizingmouseantibodies
BatchNo.Refertotheproductlabel.
AntigenThenervegrowthfactor(NGF)receptor(NGFR),alsoknownasp75neurotrophinreceptor(p75NTR;TNFRS16;CD271)isacommonreceptorfortheneurotrophinsNGF,BDNF,NT-3andNT-4/5.Inneurons,p75NTRmediatesavarietyofphysiologicalfunctionsincludingsurvival,apoptosis,neuriteoutgrowthandsynapticplasticity.Apotentialpathologicalroleforp75NTRhasbecomeevidentinrecentyears.Alteredp75NTRexpressionlevelsareimplicatedindegenerationofspinalmotorneuronsinhumanandmousemodelsofamyotrophiclateralsclerosis(ALS).Importantly,theextracellulardomainofp75NTR(p75ECD)isshedfromthecellmembraneandexcretedinurine.Recentfindingsfurthersuggestthatp75ECDcouldbeanearlybioMarkerforALSinhumans,assignificantlyelevatedp75ECDlevelsarefoundinurineofALSpatientsascomparedtohealthycontrols.
OtherNamesLow-affinitynervegrowthfactorreceptor;NGFreceptor;Gp80-LNGFR;p75ICD;Lowaffinityneurotrophinreceptorp75NTR;Tumornecrosisfactorreceptorsuperfamilymember16;CD271;NGFR;TNFRSF16.
AccessionTNR16_HUMAN
SpecificityHuman.
Cross-reactivityThisELISAkitdoesnotcross-reactwithmousep75ECDprotein.
StorageStoreat2-8°C.
ExpiryDate12monthsfrompurchase.
ReferencesThefollowingreferencesgiveageneralbackgroundonthesuitABIlityofp75ECDasabiomarkerforALSdiseaseprogression.

Shepheardetal.(2017)Neurology2017Feb22.doi:10.1212/WNL.0000000000003741.[Epubaheadofprint].

Shepheardetal.(2014)PLoSONE9(1):e87398.doi:10.1371/journal.pone.0087398.

DiStefano&Johnson(1988)ProcNatlAcadSciUSA85:270-274.
KitcomponentsTheELISAkitboxcontains2x96pre-coatedstripplates,proteinstandards,QCsample,detectionreagents,heterophilicantibodyblocker,washandsamplebuffersanddetailedprotocols.
Range7.8-500pg/mL
SensitivityTypicallimitofdetection(LOD)forhumanp75ECDislessthan6pg/mL,determinedas150%oftheblankvalue.
KitprotocolClick to download the kit protocolBEK-2219_as_at_Sep2017.pdf
MSDSClick to download the Material Safety Data SheetMSDS_Rapid_ELISA_as_at_Aug2017.pdf