Biosensis/NGFR/p75ECD Rapid ELISA Kit: Human (2 Plates)/BEK-2219-2P/1 Ea-免疫在线蚂蚁淘旗下平台

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Description	TheBiosensisNGFR/p75^ECDRapid^TMenzyme-linkedimmunosorbentassay(ELISA)KitisasandwichELISAthatallowsthequantificationoftheextracellulardomain(ECD)ofhumanp75neurotrophinreceptorinlessthan4hoursinurine,serum,citrate-plasma,CSFandcellculturesupernatantsonlyifusedasdirected.Pleaserefertothekitprotocolforspecificuseinstructionsforeachsubstrateapplication. Notethatcaptureanddetectionantibodieshavebeenoptimizedforincreasedassaysensitivity,andreagentsfrompreviouslotsshouldnotbeused.Thisoptimizedhumanp75^ECDELISAassaydemonstratesanincreasedsensitivity,loweringtheLODabout8-foldtothepreviousassayversion.ItrepresentsthemostsensitiveNGFR/p75^ECDELISAassayavailabletoday,withadetectionlimitofatleast6pg/mL,andaquantificationrangeof7.8-500pg/mL. ThisELISAkitconsistsofapre-coatedmousemonoclonalanti-p75^ECDcaptureantibody,abiotinylatedmousemonoclonalanti-p75^ECDdetectionantibodyandhorserADIshperoxidase(HRP)-conjugatedstreptavidin.Theadditionofasubstrate(3,3,5,5-tetramethylbenzidine,TMB)yieldsacoloredreactionproductwhichisdirectlyproportionaltotheconcentrationofp75^ECDpresentinsamplesandproteinstandards.Ahumanp75^ECDpositivecontrol(QCsample)isprovidedtoassureconsistentassayperformance. ThisNGFR/p75^ECDELISAkitemploysanin-houserecombinanthumanp75^ECD-Fcchimeraasstandardthathasbeenvalidatedagainstanexternallyavailablep75^ECD-Fcprotein.Whilethereisacurrentlackofacommerciallyavailable,truehumanp75^ECDstandard,thisELISAkitallowsquantificationofhumanp75^ECDasp75^ECD-Fchumanequivalents.PleasenotethattheantibodiesusedinthisELISAdonotcross-reactwithmousep75^ECD. Note1:Forresearchuseonly.Notfordiagnosticandclinicalpurposes. Note2:ThisELISAshouldnotbeconfusedwiththatreportedinthePLoSONEpublicationbyShepheardetal(2014,2017)oranyotherassay.Thiskitisuniqueandhasbeendeveloped,manufacturedandextensivelyvalidatedin-houseandisthemostsensitiveELISAavailableforthedetectionofp75NTR-ECDinhumansamples.
Relatedproducts	BEK-2220-2P,MouseNGFR/p75^ECDRapid^TMELISA BL-002-1250,HeterophilicantibodyblockerforELISAassaysutilizingmouseantibodies
BatchNo.	Refertotheproductlabel.
Antigen	Thenervegrowthfactor(NGF)receptor(NGFR),alsoknownasp75neurotrophinreceptor(p75NTR;TNFRS16;CD271)isacommonreceptorfortheneurotrophinsNGF,BDNF,NT-3andNT-4/5.Inneurons,p75NTRmediatesavarietyofphysiologicalfunctionsincludingsurvival,apoptosis,neuriteoutgrowthandsynapticplasticity.Apotentialpathologicalroleforp75NTRhasbecomeevidentinrecentyears.Alteredp75NTRexpressionlevelsareimplicatedindegenerationofspinalmotorneuronsinhumanandmousemodelsofamyotrophiclateralsclerosis(ALS).Importantly,theextracellulardomainofp75NTR(p75ECD)isshedfromthecellmembraneandexcretedinurine.Recentfindingsfurthersuggestthatp75ECDcouldbeanearlybioMarkerforALSinhumans,assignificantlyelevatedp75ECDlevelsarefoundinurineofALSpatientsascomparedtohealthycontrols.
OtherNames	Low-affinitynervegrowthfactorreceptor;NGFreceptor;Gp80-LNGFR;p75ICD;Lowaffinityneurotrophinreceptorp75NTR;Tumornecrosisfactorreceptorsuperfamilymember16;CD271;NGFR;TNFRSF16.
Accession	TNR16_HUMAN
Specificity	Human.
Cross-reactivity	ThisELISAkitdoesnotcross-reactwithmousep75^ECDprotein.
Storage	Storeat2-8°C.
ExpiryDate	12monthsfrompurchase.
References	ThefollowingreferencesgiveageneralbackgroundonthesuitABIlityofp75^ECDasabiomarkerforALSdiseaseprogression. Shepheardetal.(2017)Neurology2017Feb22.doi:10.1212/WNL.0000000000003741.[Epubaheadofprint]. Shepheardetal.(2014)PLoSONE9(1):e87398.doi:10.1371/journal.pone.0087398. DiStefano&Johnson(1988)ProcNatlAcadSciUSA85:270-274.
Kitcomponents	TheELISAkitboxcontains2x96pre-coatedstripplates,proteinstandards,QCsample,detectionreagents,heterophilicantibodyblocker,washandsamplebuffersanddetailedprotocols.
Range	7.8-500pg/mL
Sensitivity	Typicallimitofdetection(LOD)forhumanp75^ECDislessthan6pg/mL,determinedas150%oftheblankvalue.
Kitprotocol	BEK-2219_as_at_Sep2017.pdf
MSDS	MSDS_Rapid_ELISA_as_at_Aug2017.pdf