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Biosensis/NGFR/p75ECD Rapid ELISA Kit: Human (2 Plates)/BEK-2219-2P/1 Ea
Biosensis/NGFR/p75ECD Rapid ELISA Kit: Human (2 Plates)/BEK-2219-2P/1 Ea
商品编号:
BEK-2219-2P
品牌:
Biosensis inc
市场价:
¥15340.00
美元价:
9204.00
产品分类:
夹心法ELISA
公司分类:
Sandwich_method_ELISA
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Finda1platesizeofthisproducthere.Enquireaboutbulkdiscountsfortenormoreplates,atsales@Biosensis.comDescription | TheBiosensisNGFR/p75ECDRapidTMenzyme-linkedimmunosorbentassay(ELISA)KitisasandwichELISAthatallowsthequantificationoftheextracellulardomain(ECD)ofhumanp75neurotrophinreceptorinlessthan4hoursinurine,serum,citrate-plasma,CSFandcellculturesupernatantsonlyifusedasdirected.Pleaserefertothekitprotocolforspecificuseinstructionsforeachsubstrateapplication. | Notethatcaptureanddetectionantibodieshavebeenoptimizedforincreasedassaysensitivity,andreagentsfrompreviouslotsshouldnotbeused.Thisoptimizedhumanp75ECDELISAassaydemonstratesanincreasedsensitivity,loweringtheLODabout8-foldtothepreviousassayversion.ItrepresentsthemostsensitiveNGFR/p75ECDELISAassayavailabletoday,withadetectionlimitofatleast6pg/mL,andaquantificationrangeof7.8-500pg/mL. ThisELISAkitconsistsofapre-coatedmousemonoclonalanti-p75ECDcaptureantibody,abiotinylatedmousemonoclonalanti-p75ECDdetectionantibodyandhorserADIshperoxidase(HRP)-conjugatedstreptavidin.Theadditionofasubstrate(3,3,5,5-tetramethylbenzidine,TMB)yieldsacoloredreactionproductwhichisdirectlyproportionaltotheconcentrationofp75ECDpresentinsamplesandproteinstandards.Ahumanp75ECDpositivecontrol(QCsample)isprovidedtoassureconsistentassayperformance. ThisNGFR/p75ECDELISAkitemploysanin-houserecombinanthumanp75ECD-Fcchimeraasstandardthathasbeenvalidatedagainstanexternallyavailablep75ECD-Fcprotein.Whilethereisacurrentlackofacommerciallyavailable,truehumanp75ECDstandard,thisELISAkitallowsquantificationofhumanp75ECDasp75ECD-Fchumanequivalents.PleasenotethattheantibodiesusedinthisELISAdonotcross-reactwithmousep75ECD. Note1:Forresearchuseonly.Notfordiagnosticandclinicalpurposes. Note2:ThisELISAshouldnotbeconfusedwiththatreportedinthePLoSONEpublicationbyShepheardetal(2014,2017)oranyotherassay.Thiskitisuniqueandhasbeendeveloped,manufacturedandextensivelyvalidatedin-houseandisthemostsensitiveELISAavailableforthedetectionofp75NTR-ECDinhumansamples. Relatedproducts | BEK-2220-2P,MouseNGFR/p75ECDRapidTMELISA | BL-002-1250,HeterophilicantibodyblockerforELISAassaysutilizingmouseantibodies BatchNo. | Refertotheproductlabel. | Antigen | Thenervegrowthfactor(NGF)receptor(NGFR),alsoknownasp75neurotrophinreceptor(p75NTR;TNFRS16;CD271)isacommonreceptorfortheneurotrophinsNGF,BDNF,NT-3andNT-4/5.Inneurons,p75NTRmediatesavarietyofphysiologicalfunctionsincludingsurvival,apoptosis,neuriteoutgrowthandsynapticplasticity.Apotentialpathologicalroleforp75NTRhasbecomeevidentinrecentyears.Alteredp75NTRexpressionlevelsareimplicatedindegenerationofspinalmotorneuronsinhumanandmousemodelsofamyotrophiclateralsclerosis(ALS).Importantly,theextracellulardomainofp75NTR(p75ECD)isshedfromthecellmembraneandexcretedinurine.Recentfindingsfurthersuggestthatp75ECDcouldbeanearlybioMarkerforALSinhumans,assignificantlyelevatedp75ECDlevelsarefoundinurineofALSpatientsascomparedtohealthycontrols. | OtherNames | Low-affinitynervegrowthfactorreceptor;NGFreceptor;Gp80-LNGFR;p75ICD;Lowaffinityneurotrophinreceptorp75NTR;Tumornecrosisfactorreceptorsuperfamilymember16;CD271;NGFR;TNFRSF16. | Accession | TNR16_HUMAN | Specificity | Human. | Cross-reactivity | ThisELISAkitdoesnotcross-reactwithmousep75ECDprotein. | Storage | Storeat2-8°C. | ExpiryDate | 12monthsfrompurchase. | References | ThefollowingreferencesgiveageneralbackgroundonthesuitABIlityofp75ECDasabiomarkerforALSdiseaseprogression. | Shepheardetal.(2017)Neurology2017Feb22.doi:10.1212/WNL.0000000000003741.[Epubaheadofprint]. Shepheardetal.(2014)PLoSONE9(1):e87398.doi:10.1371/journal.pone.0087398. DiStefano&Johnson(1988)ProcNatlAcadSciUSA85:270-274. Kitcomponents | TheELISAkitboxcontains2x96pre-coatedstripplates,proteinstandards,QCsample,detectionreagents,heterophilicantibodyblocker,washandsamplebuffersanddetailedprotocols. | Range | 7.8-500pg/mL | Sensitivity | Typicallimitofdetection(LOD)forhumanp75ECDislessthan6pg/mL,determinedas150%oftheblankvalue. | Kitprotocol | BEK-2219_as_at_Sep2017.pdf | MSDS | MSDS_Rapid_ELISA_as_at_Aug2017.pdf | 品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
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TR-200-BG型
PE-1243-50型
抗体试剂盒
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电话:PE-1239-5
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B-100-PS型
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2个平板ELISA试剂盒
M-1763-100英尺
TR-200-BGT公司
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PE-1242-5型
M-1249-100号
神经营养因子受体
细胞周期调节器
S-1759-100号
PE-1606-100型
PE-1762-100型
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PE-019-100型
PE-1241-5型
PE-003-100
1个平板ELISA试剂盒
BL-003-1000型
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感觉神经系统
PE-1253-100型
TR-100-FJ
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神经内分泌
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PE-1243-25
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神经递质和酶
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标记抗体
BL-004-500型
PE-019-25
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受体体嵌合体
信令与转录
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BL-001-1250型
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阿尔茨海默氏症/帕金森氏症
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