Biosensis/NGFR/p75ECD Rapid ELISA Kit: Mouse (2 Plates)/BEK-2220-2P/1 Ea-免疫在线蚂蚁淘旗下平台

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商品详细Biosensis/NGFR/p75ECD Rapid ELISA Kit: Mouse (2 Plates)/BEK-2220-2P/1 Ea

Biosensis/NGFR/p75ECD Rapid ELISA Kit: Mouse (2 Plates)/BEK-2220-2P/1 Ea

商品编号： BEK-2220-2P

品牌： Biosensis inc

市场价： ¥15340.00

美元价： 9204.00

产地：美国(厂家直采)

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产品分类：夹心法ELISA

公司分类： Sandwich_method_ELISA

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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	Find a 1 plate size of this product here. Enquire about bulk discounts for ten or more plates, at sales@biosensis.com
Description	The Biosensis NGFR/p75^ECD Rapid^TM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of mouse p75^ECD in less than 4 hours n cell culture supernatants and urine only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application. This ELISA kit consists of a pre-coated mouse monoclonal anti-p75^ECD capture antibody, a goat anti- p75^ECD detection antibody and a horseradish peroxidase (HRP)-conjugated anti-goat antibody. The addition of a substrate (3,3,5,5-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of p75ECD present in samples and protein standards. This NGFR/p75^ECD ELISA kit employs a recombinant mouse p75^ECD-Fc chimera protein as standard. While there is a current lack of a true mouse p75^ECD standard, this ELISA kit allows quantification of mouse p75^ECD as p75^ECDFc mouse equivalents. Please note that the antibodies used in this ELISA cross-react with human NGFR/p75^ECD. This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures. Note: The Biosensis NGFR/p75^ECD Rapid^TM ELISA is not identical to the ELISA reported in Shepheard et al. (2014) in PLoS ONE.
Related products	BEK-2219-2P, Human NGFR/p75^ECD Rapid^TM ELISA
Batch No.	Refer to the product label.
Antigen	The nerve growth factor (NGF) receptor (NGFR), also known as p75 neurotrophin receptor (p75NTR; TNFRS16; CD271) is a common receptor for the neurotrophins NGF, BDNF, NT-3 and NT-4/5. In neurons, p75NTR mediates a variety of physiological functions including survival, apoptosis, neurite outgrowth and synaptic plasticity. A potential pathological role for p75NTR has become evident in recent years. Altered p75NTR expression levels are implicated in degeneration of spinal motor neurons in human and mouse models of amyotrophic lateral sclerosis (ALS). Importantly, the extracellular domain of p75NTR (p75ECD) is shed from the cell membrane and excreted in urine. Recent findings further suggest that p75ECD could be an early biomarker for ALS in humans, as significantly elevated p75ECD levels are found in urine of ALS patients as compared to healthy controls.
Other Names	Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR; Tumor necrosis factor receptor superfamily member 16; CD271; NGFR; TNFRSF16.
Accession	TNR16_MOUSE
Specificity	Mouse.
Cross-reactivity	The antibodies used in this ELISA kit are known to cross-react with human p75ECD protein.
Storage	Store at 2-8°C.
Expiry Date	12 months from purchase.
Specific References	Maejima H, Kanemura N, Kokubun T, Murata K, Takayanagi K (2017) Exercise enhances cognitive function and neurotrophin expression in the hippocampus accompanied by changes in epigenetic programming in senescence-accelerated mice. Neurosci Lett. doi: 10.1016/j.neulet.2017.11.023. [Epub ahead of print]. Application: Mouse hippocampus homogenates.
General References	DiStefano & Johnson (1988) Proc Natl Acad Sci USA 85: 270-274. Shepheard et al. (2014) PLoS ONE 9(1): e87398. doi:10.1371/journal.pone.0087398.
Kit components	The ELISA kit box contains 2 x 96 pre-coated strip plates, protein standards, detection reagents, wash and sample buffers and detailed protocols.
Range	62.5 - 4000 pg/mL
Sensitivity	This ELISA kit typically detects 20-40 pg/mL of mouse p75ECD (defined as blank OD plus 3x the standard deviation of the blank OD, n=10).
Kit protocol	BEK-2220_as_at_Jan2017.pdf
MSDS	MSDS_Rapid_ELISA_as_at_Aug2017.pdf

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