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当前位置: 首页 > 产品中心 > Receptors > Biosensis/Human TrkA-Fc Chimera expressed in mammalian cells/PE-1248-50/50 µg
商品详细Biosensis/Human TrkA-Fc Chimera expressed in mammalian cells/PE-1248-50/50 µg
Biosensis/Human TrkA-Fc Chimera expressed in mammalian cells/PE-1248-50/50 µg
Biosensis/Human TrkA-Fc Chimera expressed in mammalian cells/PE-1248-50/50 µg
商品编号: PE-1248-50
品牌: Biosensis inc
市场价: ¥4540.00
美元价: 2724.00
产地: 美国(厂家直采)
公司:
产品分类: 受体
公司分类: Receptors
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionTrkAisamemberoftheneurotrophictyrosinekinasereceptorfamily.Itisamembrane-boundreceptorthatuponneurotrophinbinding,phosphorylatesitselfandmembersoftheMAPKpathway.TrkAisrequiredforhigh-affinitybindingtonervegrowthfactor(NGF),neurotrophin-3andneurotrophin-4/5butnotbrain-derivedneurotrophicfactor(BDNF).TrkAleadstocelldifferentiationsandmayplayaroleinspecifyingsensoryneuronsubtypes.Ithasacrucialroleinthedevelopmentandfunctionofthenociceptivereceptionsystemaswellasestablishmentofthermalregulationviasweating.SUBUNIT:Existsinadynamicequilibriumbetweenmonomeric(lowaffinity)anddimeric(highaffinity)structures.SUBCELLULARLOCATION:TrkAisaheavilyglycosylatedtransmembraneproteinandcontains13potentialN-linkedglycosylationsites.TheTrkA-FcchimerahasN-linkedandmayhaveO-linkedoligosaccharides.
BatchNo.Seeproductlabel
Unitsize50µg
OtherNamesTropomyosin-relatedkinasereceptor;Highaffinitynervegrowthfactorreceptor;Neurotrophictyrosinekinasereceptortype1;TRK1transformingtyrosinekinaseprotein;p140-TrkA;Trk-A;NTRK1;TRK
AccessionP04629NTRK1_HUMAN;
ProducedinHuman-ADNAsequenceencodingthesignalpeptideandextracellulardomainofhumanTrkA(aminoacids1-413)wasfusedtotheFcregionofhumanIgG1(aminoacids104-330).Thechimericproteinwasexpressedinmodifiedhuman293cells.
MolecularWeightTheTrkA-Fcchimeramigratesasabroadbandbetween90and120kDainSDS-PAGEunderreducingconditionsduetopost-translationalmodifications,inparticularglycosylation.TheunmodifiedTrkA-Fcchimerathathasapredictedmolecularmassof70.2kDaandpredictedpIof5.8.Undernon-reducingconditions,TrkA-Fcmigratesasabroadbandbetween180and240kDademonstratingtheexistenceofthisproteinasadimer.Asecond,lessintensebandisobservedat140kDaundertheseSDS-PAGEconditions,suggestingthepresenceofnon-glycosylatedTrkA-Fcisoforms.
Purity>95%,asdeterminedbySDS-PAGE.
FormProteinGpurified.
ReconstitutionSpinvialbrieflybeforeopening.Reconstitutein100uLsterilePBS,pH7.4togiveaconcentrationof0.5mg/mL.
StorageLyophilizedproductshouldbestoredat2to8°C.Followingreconstitution,short-termstorageat4°Cisrecommendedandlonger-termstorageofaliquotsat-18to-20°C.Repeatedfreezethawingisnotrecommended.
品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
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