Biosensis/Amylo-Glo-RTD淀粉样斑块染色试剂和EtBr计数器染色/TR-400-AG/1 Ea
商品编号:
TR-400-AG
品牌:
Biosensis inc
市场价:
¥5940.00
美元价:
3564.00
产品分类:
其他试剂
公司分类:
Other_reagents
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Description | TheBiosensisAG-400-AGkitutilizesanethidiumbromidecounterstainforaquickandeffectivewaytovisualizecellnucleiandcellbodiesofcellswhileunderUVIlluminationallowingtheassessmentofamyloidplaquesandcell/tissuepositioningaswellinonestep. Amylo-Glo®RTDReadytoDiluteStainingreagentisdesignedtostainamyloidplaquesintissuesections.ThisnovelMarkerhasseveraladvantagesoverotherconventionalmarkerssuchasThioflavinSandCongoRedbecauseofitsuniquechemicalandspectralproperties.(L.Schmuedetal.(2012)J.NeuroscienceMethods209:120126). UsingAmylo-Glo®resultsinaverybrightblueUVexcitablestainunderphysiologicalconditionsthatwillnotbleedthroughwhenilluminatedwithotherfilters.Itsbrightnessmakesitidealforlowmagnificationquantificationstudies,whileitsuniqueexcitation/emissionprofileandmildstainingconditionsmakesitidealforcombinationformultipleimmunofluorescentlabelingstudies.Amylo-Glo®RTDiscompatIBLewithfresh,frozen,andformalin-fixedimmunohistochemistryorcytochemistry,anditisparticularlygoodforconfocalandmultiplelabelingbecauseofitshighfluorescentintensityandhighresistancetophoto-bleaching.MoreoverbecauseAmylo-Glo®fluorescesintheUVchannel,doubleandtriplelabelingexperimentscanbeperformedveryeasily(seeprotocol). |
Relatedproducts | TR-300-AG,MOAB-2antibody |
BatchNo. | seeproductlabel |
OtherNames | AmyloGlo |
CompoundName | Compound:Amylo-Glo;Classification:Styrylbenzenederivative;Appearance:Yellowsolution;MolecularWeight:392;Filtersystemforvisualizing:UVEthibiumBromide:EtBr,2,7-Diamino-10-ethyl-6-phenylphenanthridiniumbromide;Appearance:lightred-orangesolution;MolecularWeight:394.32.392;Filtersystemforvisualizing:UV |
Purity | Thinlayerchromatographyusingaluminaplatesandasolventsystemofethanolandwater(3:1)revealedthepresenceoftwofluorescentisomers.Noamountofstartingmaterialwasdetected. |
Biol.activity | ExcitationPeakforAmylo-Glo:334EmissionPeak:533nm-unbound,438nmwhenboundtoamyloid.EtBr:Ethidiumbromidehasanexcitationpeakof300nmandanemissionpeak595nm. |
Applications | Stainingofamyloidplaquesinhumanandanimaltissues,seeincludedprotocol.EtBrcounterstainstainsnucleiandcellbodiesforeasyidentificationandspacialorientation. |
Specificity | amyloidplaquesbothintraneuronalandvascularforA-G,Etbr,nucleiandcellbodiesbothDNAandRNAlabel |
Appearance | ExcitationPeak:334;EmissionPeak:533nm-unbound,438nmwhenboundtoamyloid.TovisualizeAmylo-glointissue,UVlightisrequired.Forexample,Amylo-GlotissuecanbeexaminedusinganepifluoresentmicroscopewithUV(NikonUV-2A)filtercube.Excitation(325-375nm)Emission(400-450nm)istypical.Alsonote,itisnotuncommonforAmylo-Glotoappearlightyellowwhenexaminedbyeye,yetappearalightbluecolorwhenphotographed. VisualizationofEtBr:Ethidiumbromidehasanexcitationpeakof300nmandanemissionpeak595nm.MostUVcompatiblefiltersetscanbeused. |
Reconstitution | Readytodiluteperprotocol10Xsolutions |
Storage | Thestocksolutioncanbestoredforupto6monthsat4°Cprotectedfromlight.Nopreservatives.Usesteriletechniquewhenhandlingandproperlaboratoryprocedures. |
ExpiryDate | 6monthsfromdateofpurchase |
GeneralReferences | L.Schmuedetal.(2012)J.NeuroscienceMethods209:120126 |
KitComponents | 1bottlecontaining40mlof10XAmylo-Glo®RTD(A-GRTD)solution1bottlecontaining40mlof10XA-GRTDEthidiumBromide(EtBrRTD)solution |
ReagentKitprotocol | TR-400-AG_as_at_Apr2014_protocol.pdf |
MSDS | MSDS_TR-400-AG_as_at_July2015.pdf |
品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
品牌分类
TR-200-BG型
PE-1243-50型
抗体试剂盒
PE-1246-5型
TR-100-BG型
S-1754-500型
神经营养因子
糖尿病与肥胖
细胞内贩运
电话:PE-1239-5
神经肽和受体
B-100-PS型
受体和通道分子
2个平板ELISA试剂盒
M-1763-100英尺
TR-200-BGT公司
电话:PE-1239-2
PE-1242-5型
M-1249-100号
神经营养因子受体
细胞周期调节器
S-1759-100号
PE-1606-100型
PE-1762-100型
B-200-PS型
PE-019-100型
PE-1241-5型
PE-003-100
1个平板ELISA试剂盒
BL-003-1000型
PE-1246-2型
感觉神经系统
PE-1253-100型
TR-100-FJ
BL-005-500型
神经内分泌
M-1728-100型
DNA损伤与修复
PE-001-100型
PE-1608-1型
TR-400-AG公司
PE-1243-25
细胞标记
PE-1608-5型
TR-100-FJT
自噬
PE-1242-2型
其他神经元
神经递质和酶
抗氧化酶
神经元标志物
标记抗体
BL-004-500型
PE-019-25
PE-1608-10型
BL-002-1250型
受体体嵌合体
信令与转录
基质和粘附分子
BL-001-1250型
癌症
PE-019-500
干细胞
连接蛋白
TR-300-AG型
细胞凋亡
PE-1252-100型
生长因子
发展
阿尔茨海默氏症/帕金森氏症
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