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当前位置: 首页 > 产品中心 > Other_reagents > Biosensis/Amylo-Glo-RTD淀粉样斑块染色试剂和EtBr计数器染色/TR-400-AG/1 Ea
商品详细Biosensis/Amylo-Glo-RTD淀粉样斑块染色试剂和EtBr计数器染色/TR-400-AG/1 Ea
Biosensis/Amylo-Glo-RTD淀粉样斑块染色试剂和EtBr计数器染色/TR-400-AG/1 Ea
Biosensis/Amylo-Glo-RTD淀粉样斑块染色试剂和EtBr计数器染色/TR-400-AG/1 Ea
商品编号: TR-400-AG
品牌: Biosensis inc
市场价: ¥5940.00
美元价: 3564.00
产地: 美国(厂家直采)
公司:
产品分类: 其他试剂
公司分类: Other_reagents
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionTheBiosensisAG-400-AGkitutilizesanethidiumbromidecounterstainforaquickandeffectivewaytovisualizecellnucleiandcellbodiesofcellswhileunderUVIlluminationallowingtheassessmentofamyloidplaquesandcell/tissuepositioningaswellinonestep.

Amylo-Glo®RTD™“ReadytoDilute”Stainingreagentisdesignedtostainamyloidplaquesintissuesections.ThisnovelMarkerhasseveraladvantagesoverotherconventionalmarkerssuchasThioflavinSandCongoRedbecauseofitsuniquechemicalandspectralproperties.(L.Schmuedetal.(2012)J.NeuroscienceMethods209:120–126).  UsingAmylo-Glo®resultsinaverybrightblueUVexcitablestainunderphysiologicalconditionsthatwillnotbleedthroughwhenilluminatedwithotherfilters.Itsbrightnessmakesitidealforlowmagnificationquantificationstudies,whileitsuniqueexcitation/emissionprofileandmildstainingconditionsmakesitidealforcombinationformultipleimmunofluorescentlabelingstudies.Amylo-Glo®RTD™iscompatIBLewithfresh,frozen,andformalin-fixedimmunohistochemistryorcytochemistry,anditisparticularlygoodforconfocalandmultiplelabelingbecauseofitshighfluorescentintensityandhighresistancetophoto-bleaching.MoreoverbecauseAmylo-Glo®fluorescesintheUVchannel,doubleandtriplelabelingexperimentscanbeperformedveryeasily(seeprotocol).
RelatedproductsTR-300-AG,MOAB-2antibody
BatchNo.seeproductlabel
OtherNamesAmyloGlo
CompoundNameCompound:Amylo-Glo;Classification:Styrylbenzenederivative;Appearance:Yellowsolution;MolecularWeight:392;Filtersystemforvisualizing:UVEthibiumBromide:EtBr,2,7-Diamino-10-ethyl-6-phenylphenanthridiniumbromide;Appearance:lightred-orangesolution;MolecularWeight:394.32.392;Filtersystemforvisualizing:UV
PurityThinlayerchromatographyusingaluminaplatesandasolventsystemofethanolandwater(3:1)revealedthepresenceoftwofluorescentisomers.Noamountofstartingmaterialwasdetected.
Biol.activityExcitationPeakforAmylo-Glo:334EmissionPeak:533nm-unbound,438nmwhenboundtoamyloid.EtBr:Ethidiumbromidehasanexcitationpeakof300nmandanemissionpeak595nm.
ApplicationsStainingofamyloidplaquesinhumanandanimaltissues,seeincludedprotocol.EtBrcounterstainstainsnucleiandcellbodiesforeasyidentificationandspacialorientation.
SpecificityamyloidplaquesbothintraneuronalandvascularforA-G,Etbr,nucleiandcellbodiesbothDNAandRNAlabel
AppearanceExcitationPeak:334;EmissionPeak:533nm-unbound,438nmwhenboundtoamyloid.TovisualizeAmylo-glointissue,UVlightisrequired.Forexample,Amylo-GlotissuecanbeexaminedusinganepifluoresentmicroscopewithUV(NikonUV-2A)filtercube.Excitation(325-375nm)Emission(400-450nm)istypical.Alsonote,itisnotuncommonforAmylo-Glotoappearlightyellowwhenexaminedbyeye,yetappearalightbluecolorwhenphotographed.
VisualizationofEtBr:Ethidiumbromidehasanexcitationpeakof300nmandanemissionpeak595nm.MostUVcompatiblefiltersetscanbeused.
ReconstitutionReadytodiluteperprotocol10Xsolutions
StorageThestocksolutioncanbestoredforupto6monthsat4°Cprotectedfromlight.Nopreservatives.Usesteriletechniquewhenhandlingandproperlaboratoryprocedures.
ExpiryDate6monthsfromdateofpurchase
GeneralReferencesL.Schmuedetal.(2012)J.NeuroscienceMethods209:120–126
KitComponents1bottlecontaining40mlof10XAmylo-Glo®RTD™(A-GRTD™)solution1bottlecontaining40mlof10XA-GRTD™EthidiumBromide(EtBrRTD™)solution
ReagentKitprotocolClick to download the protocolTR-400-AG_as_at_Apr2014_protocol.pdf
MSDSClick to download the Material Safety Data SheetMSDS_TR-400-AG_as_at_July2015.pdf
品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
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