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当前位置: 首页 > 产品中心 > Other_reagents > Biosensis/Black Gold II髓鞘稀释染色试剂盒,甲苯胺蓝O计数器染色,用于识别正常和路径
商品详细Biosensis/Black Gold II髓鞘稀释染色试剂盒,甲苯胺蓝O计数器染色,用于识别正常和路径
Biosensis/Black Gold II髓鞘稀释染色试剂盒,甲苯胺蓝O计数器染色,用于识别正常和路径
Biosensis/Black Gold II髓鞘稀释染色试剂盒,甲苯胺蓝O计数器染色,用于识别正常和路径
商品编号: TR-100-BG
品牌: Biosensis inc
市场价: ¥11940.00
美元价: 7164.00
产地: 美国(厂家直采)
公司:
产品分类: 其他试剂
公司分类: Other_reagents
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionBlack-Gold®IIisanovelhaloaurophosphatecomplexwhichlocalisesmyelinwithinthecentralnervoussystem.TheBlackGoldIIReady-to-Dilute(RTD™)StainingKitallowsyoutolocalisemyelin,bothindividualfibresandtracts,alongwiththeoptionofco-localisingcellbodiesviatheToluidineBluecounterstain.BlackGoldIIlabelledmyelinatedfibresappearnearlyblackwhiletheToluidineBlueOlabelledcellularNisslbodiesareblueunderbrightfieldIllumination.

BlackGoldIIcandemonstrateandcharacterisespecificmyelinchangesassociatedwithexposuretodiverseneurotoxicantsincludingkainicacid,domoicacid,3-nitropropionicacid,Fluoro-Goldandisoniazid.BlackGoldIIcanalsobecombinedwithotherhistochemicalMarkersincludingNisslstains,retrogradelytransportedfluorescenttracersandfluorescentmarkersofneuronaldegeneration.TheadvantagesassociatedwiththeBlack-GoldIIincludehighresolution,highcontrast,shorthistochemicalprocessingtime,versatilityandconsistentreproducibility.
OtherNamesblackandgold
ApplicationsBlackGoldIIisahighresolutionmyelinstainwithamyloidplaquecounterstain.Itsuseistailoredtostudiesusingformalinorparaformaldehydefixed,non-paraffinembedded,non-solventprocessedbraintissue.Itcanbeusedwithboththickandthinsections.Forthicksections,gelatincoatedslidesorslidesspeciallydesignedtobindtissuessectionsshouldbeusetoavoidsectionloss.Free-floatingsectionscanbeusedaswellbutsectionsareeasiertohandleandtransferwhenmountedonslides.Asuggestedmethodforthicksectionsisprovidedasaguide:Eitherfrozenorvibratomesectionsarecutatathicknessof20-50umandcollectedin0.1Mneutralphosphatebuffer.Thesectionsarethentypicallymountedon1%gel-coatedslidesandthenairdriedonaslidewarmer(at50degreesC)foratleastanhouruntilthroughlydriedandadheredtotheslide.Thesectionscanbestainedloose,althoughthesectionsareeasiertohandlewhenmountedonslides.Themountedsectionswererehydratedindistilledwaterfor2minutesbeforebeingprocessedinthestainingsolutions.
FormThereagentsintheBlackGoldkitareallsuppliedinaliquidformatandareready-to-dilute.
StorageThekitcanbetransportedatroomtemperature.Oncereceived,thekitcanbestoredforupto12monthsat4°Cprotectedfromlight.Dilutedsolutionscanbestoreduptoonemonthat4°Cprotectedfromlight.
SpecificReferencesYingYLetal.(2014)Adultneuralprecursorcellsfromthesubventricularzonecontributesignificantlytooligodendrocyteregenerationandremyelination.JNeurosci.2014Oct15;34(42):14128-46
KitComponentsBlack-GoldII(Dilute1:10priortouse)-10mLSodiumThiosulfate,fixative(Dilute1:10priortouse)-10mLToluidineBlueO(Dilute1:10priortouse)-10mLAceticAcid(Dilute1:10priortouse)-10mL
ReagentKitprotocolClick to download the protocolTR-100-BG_April2012.pdf
MSDSClick to download the Material Safety Data SheetMSDS_TR-100-BG_BGT_as_at_Apr2014.pdf
品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
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