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Biosensis/Human Soluble Vascular Endothelial Growth Factor receptor 1 / sVEGFR1 ELISA Kit (1 plate)/
来自 : 发布时间:2024-05-17
Find a 2 plate size of this product here. Enquire about bulk discounts for ten or more plates, at sales@biosensis.com
Description The human Vascular Endothelial Growth Factor receptor 1 (VEGFR1) Kit is a sandwich ELISA. The capture antibody is a monoclonal human VEGFR1 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known VEGFR1 concentration are added to these wells and allowed to complex with the bound VEGFR1 antibody. A biotinylated anti-human VEGFR1 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of VEGFR1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human VEGFR1 in samples such as sera, plasma, and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Batch No. See product labels
Antigen Recombinant sVEGFR1 amino acids 27-687 (located in the ECD domain region) produced in and purified from a sf21 insect culture system.
Sequence 30 40 50 27-SKLK DPELSLKGTQ HIMQAGQTLH 60 70 80 90 100LQCRGEAAHK WSLPEMVSKE SERLSITKSA CGRNGKQFCS TLTLNTAQAN 110 120 130 140 150HTGFYSCKYL AVPTSKKKET ESAIYIFISD TGRPFVEMYS EIPEIIHMTE 160 170 180 190 200GRELVIPCRV TSPNITVTLK KFPLDTLIPD GKRIIWDSRK GFIISNATYK 210 220 230 240 250EIGLLTCEAT VNGHLYKTNY LTHRQTNTII DVQISTPRPV KLLRGHTLVL 260 270 280 290 300NCTATTPLNT RVQMTWSYPD EKNKRASVRR RIDQSNSHAN IFYSVLTIDK 310 320 330 340 350MQNKDKGLYT CRVRSGPSFK SVNTSVHIYD KAFITVKHRK QQVLETVAGK 360 370 380 390 400RSYRLSMKVK AFPSPEVVWL KDGLPATEKS ARYLTRGYSL IIKDVTEEDA 410 420 430 440 450GNYTILLSIK QSNVFKNLTA TLIVNVKPQI YEKAVSSFPD PALYPLGSRQ 460 470 480 490 500ILTCTAYGIP QPTIKWFWHP CNHNHSEARC DFCSNNEESF ILDADSNMGN 510 520 530 540 550RIESITQRMA IIEGKNKMAS TLVVADSRIS GIYICIASNK VGTVGRNISF 560 570 580 590 600YITDVPNGFH VNLEKMPTEG EDLKLSCTVN KFLYRDVTWI LLRTVNNRTM 610 620 630 640 650HYSISKQKMA ITKEHSITLN LTIMNVSLQD SGTYACRARN VYTGEEILQK 660 670 680 KEITIRDQEA PYLLRNLSDH TVAISSSTTL DCHANGV-687
Other Names vascular permeability factor receptor; Tyrosine-protein kinase receptor FLT; Tyrosine-protein kinase FRT; Fms-like tyrosine kinase 1; VEGFR-1; FLT1;
Accession P17948 VGFR1_HUMAN;
Produced in sf-21 insect cell culture
Molecular Weight Standard runs as 100 kDa on rPAGE
Applications ELISA
Specificity Human Vascular Endothelial Growth Factor receptor 1
Cross-reactivity Human sVEGFR1 isoforms
Storage Store at 4øC
Kit components The ELISA kit box contains 1 x 96 pre-coated strip plates, protein standards, detection reagents, substrate buffer and precise instructions.
Range 156 pg/ml - 10,000 pg/ml
Sensitivity < 30 pg/ml
Kit protocol \"ClickBEK-2162-1P_as_at_Sept2015_protocol.pdf

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发布于 : 2024-05-17 阅读()
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