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Biosensis/Rat Interleukin 10 ELISA Kit (1 plate)/BEK-2047-1P/1 Ea
用于BEK-2211和类似ELISA分析的Biosensis/分泌型IgA阻滞剂/BL-001-1250/1250µg
Biosensis/兔抗人BNIP3（38-53）多克隆抗体：亲和纯化/R-1597-100/100µg
Biosensis/Mouse monoclonal antibody to human copine 6 [6D11]: IgG/M-816-100/100 µg
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Biosensis/proNGF Rapid ELISA Kit: Human (2 plates)/BEK-2226-2P/1 Ea
来自 : 发布时间：2024-11-01
Find a 1 plate size of this product here. Enquire about bulk discounts for ten or more plates, at sales@Biosensis.com
Description The Biosensis proNGF RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of full-length proNGF protein in less than 4 hours in human serum, heparin-plasma, cell supernatants and lysates only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application.This ELISA kit consists of a pre-coated anti-proNGF capture antibody, a biotinylated anti-proNGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3’,5,5’-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proNGF present in samples and protein standards. A human proNGF positive control (QC sample) is provided to assure consistent assay performance.This proNGF ELISA kit contains a recombinant human proNGF standard expressed in E.coli. This ELISA kit does not cross-react with the mouse form of proNGF, and due to sequence homology of mouse and rat proNGF is not expected to detect rat proNGF. The antibodies used in this ELISA kit bind epitopes within the pro-domain of the protein and therefore recognize proNGF and the pro-domain peptide, but do not cross-react with mature NGF! Note that accurate proNGF quantification in human serum requires the addition of Heterophilic Antibody Blocker BL-003-1000 provided in the kit, and available for purchase separately.This kit has not been tested for other applications. Sufficient amount of proNGF standard is supplied to allow for spike- and recovery experiments in order to validate this ELISA assay for other sample matrices if required. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Related products BL-003-1000, Heterophilic antibody blocker: Sample diluent additive for accurate quantification or proNGF in human serum.
Batch No. Refer to the product label.
Antigen Nerve growth factor (NGF) is synthesized as a precursor (proNGF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proNGF is synthesized in target tissues and glia, transported retrogradely and may be released.
Other Names pro-brain nerve growth factor; proNGF; NGF
Accession NGF_HUMAN
Specificity Human. Does not react with mouse proNGF, and is not expected to detect rat proNGF.
Cross-reactivity Does not cross-react with recombinant human NGF and proBDNF tested at 25 ng/mL.
Storage Store at 4°C.
Expiry Date 12 months from purchase.
Kit components The ELISA kit box contains 2 x 96 pre-coated strip plates, protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers and detailed protocols.
Range 0.078 - 5 ng/mL
Sensitivity Typical limit of detection (LOD) for proNGF is 30-60 pg/mL determined as blank value plus 3x standard deviation of blank OD (n=10).
Kit protocol \"ClickBEK-2226_as_at_Sep2017.pdf
MSDS \"ClickMSDS_Rapid_ELISA_as_at_Aug2017.pdf

本文链接： http://biosensis.immuno-online.com/view-1518595832.html

发布于 ： 2024-11-01 阅读（）
最新动态
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Biosensis/Mouse ICAM-1 ELISA Kit (1 plate)/BEK-2024-1P/1 Ea 2020-09-17
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Biosensis/Human Soluble Vascular Endothelial Growth Factor receptor 1 / sVEGFR1 ELISA Kit (1 plate)/ 2021-05-10
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公司介绍
Biosensis是一家由生命科学家和商业团队组成的公司，致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验，并在生命科学研究和诊断市场的生命科学抗体，试剂和试剂盒的商业化方面拥有50多年的经验。
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TR-200-BG型 PE-1243-50型 抗体试剂盒 PE-1246-5型 TR-100-BG型 S-1754-500型 神经营养因子 糖尿病与肥胖 细胞内贩运
电话：PE-1239-5 神经肽和受体 B-100-PS型 受体和通道分子 2个平板ELISA试剂盒 M-1763-100英尺 TR-200-BGT公司 电话：PE-1239-2 PE-1242-5型 M-1249-100号 神经营养因子受体 细胞周期调节器 S-1759-100号 PE-1606-100型 PE-1762-100型 B-200-PS型 PE-019-100型 PE-1241-5型 PE-003-100 1个平板ELISA试剂盒 BL-003-1000型 PE-1246-2型 感觉神经系统 PE-1253-100型 TR-100-FJ BL-005-500型 神经内分泌 M-1728-100型 DNA损伤与修复 PE-001-100型 PE-1608-1型 TR-400-AG公司 PE-1243-25 细胞标记 PE-1608-5型 TR-100-FJT 自噬 PE-1242-2型 其他神经元 神经递质和酶 抗氧化酶 神经元标志物 标记抗体 BL-004-500型 PE-019-25 PE-1608-10型 BL-002-1250型 受体体嵌合体 信令与转录 基质和粘附分子 BL-001-1250型 癌症 PE-019-500 干细胞 连接蛋白 TR-300-AG型 细胞凋亡 PE-1252-100型 生长因子 发展 阿尔茨海默氏症/帕金森氏症
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