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Biosensis/Mouse Osteopontin ELISA Kit (2 plates)/BEK-2084-2P/1 Ea
来自 : 发布时间:2024-11-01
Find a 1 plate size of this product here. Enquire about bulk discounts for ten or more plates, at sales@biosensis.com
Description The mouse Osteopontin (OPN) Kit is a sandwich ELISA. The capture antibody is a polyclonal mouse OPN antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known OPN concentration are added to these wells and allowed to complex with the bound OPN antibody. A biotinylated mouse OPN polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of OPN present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse OPN in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Batch No. See product labels
Other Names OPN; 2AR; Bone sialoprotein 1; Calcium oxalate crystal growth inhibitor protein;Early T-lymphocyte activation 1 protein; Minopontin; Secreted phosphoprotein 1; SPP-1;Eta-1; Op; Spp1;
Accession P10923 OSTP_MOUSE;
Specificity Mouse OPN
Storage Store at 4°C
Kit components The ELISA kit box contains 2 x 96 pre-coated strip plates, protein standards, detection reagents, substrate buffer and precise instructions.
Range 156 pg/ml - 10,000 pg/ml
Sensitivity < 10 pg/ml
Kit protocol \"ClickBEK-2084-2P_as_at_Jun2014_protocol.pdf

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发布于 : 2024-11-01 阅读()
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